The purpose of this study is to determine the enzyme composition and characteristics of malignant cells in retinoblastoma tissue. Retinal cells from normal (human eyes enucleated for various reasons other than for diseases of the retina) and malignant retinoblastoma tissue, will be sectioned immediately after enucleation to obtain viable retinal tissue. The retina will be used either directly, to identify specific enzymes and their concentration, or will be grown in cell culture for enzyme analysis after a few passages. An effort will be made to clone out a malignant cell line of retinoblastoma for enzyme and chromosomal analysis. Chromosome studies will be made on original malignant tissue and on peripheral lymphocytes from retinoblastoma cases and closely related family members. Cell lines of retinoblastoma will be further examined for cytotoxic effects of currently available metabolic inhibitors and immune globulins prepared against specific tumor antigens.